澳洲本科申请过程:如何对分光光度计对药物进行定量

14 5月 澳洲本科申请过程:如何对分光光度计对药物进行定量

澳洲本科申请过程:如何对分光光度计对药物进行定量

这一过程的第一步包括在分光光度计的帮助下对药物进行定量。它是在透明溶剂中进行的,吸光度是在给定波长下测量的。通常选择的波长是属于最大吸收波长(λmax)。在这个步骤中,在波长的确定过程中遇到的一个小误差对吸光度的测量值的影响最小。在下一步中,浓度的调整应使吸光度在0.9左右。在这一步中,计算由多种吸收物质组成的单一组分的含量(Behera et al., 2012)。这种计算可以用标准吸光度值、校准图或单点或双点标准化来完成。在标准值吸收法中,用标准值A或E来测定吸光度。这种方法被证明是有用的,当很难获得任何具体的样品的物质。
在校准图法中,有一种对标准溶液的测量,该标准溶液的浓度将包含样品。然后,对标定图进行了构造。浓度是在校准图本身的帮助下读取的。在单点标准化过程中,需要对样品溶液的吸光度进行测量,并对另一标准溶液的吸光度进行测量。利用吸光度与浓度的比例关系来计算物质的浓度。用于研究目的的材料是扑热息痛。它是一种药物,是一种主要用于药物基质的非活性成分。为了进行对乙酰氨基酚分析的相关实验,使用了甲醇和水的稀释剂。约10mg的药物溶于15ml量的甲醇l中。加入85 ml的水,使100 ml的完整溶液。从该溶液中取5 ml左右,用稀释剂将体积调节到50 ml左右。

澳洲本科申请过程:如何对分光光度计对药物进行定量

The very first step involved in this process includes the quantification of the medicinal substance with the help of a spectrophotometer. It is done in a transparent solvent and the absorbance is measured at a given wavelength. The wavelength which is generally selected is the wavelength belonging to the maximum absorption (λmax). In this step, a small error when encountered in the settling of the wavelength would have a minimum impact on the measured value of absorbance. In the next step, the concentration should be adjusted in such a manner that there is an absorbance of around 0.9. In this step, the calculation of the assay of a single component is carried out which consists of many other kinds of absorbing substances (Behera et al., 2012). This calculation can be done either with the use of standard absorptivity value, calibration graph or the single or the double point standardization. In the standard value absorptive method, standard A or E Values are used for doing the determination of the absorptiveness. This method proves to be useful when it is difficult to obtain any specific sample of the substance.
In calibration graph method, there is a measurement of the standard solution of the reference substance which are present at a concentration that would encompass he sample. After this, the construction of the calibration graph is done. The concentration is read with the help of the calibration graph itself. In single point standardization procedure, there is a measurement of absorbance of the sample solution and the measurement of another standard solution which is made of the reference substance.The concentration of the substances is calculation with the help of the proportional relationship between absorbance and the concentration. The material to be used for the purpose of research is Paracetamol. It is a drug and an inactive ingredient which is largely used in drug matrix. In order to do the experiment related to the analysis of paracetamol, diluents of methanol and water is used. Around 10 mg of drug is dissolved in 15 ml quantity of methanol l.85 ml of water is added to make the complete solution of 100 ml. From this solution, around 5 ml is taken and the volume is adjusted to around 50 ml with diluents.